The following services are available and chargeable to the investigators:

Service:

Sample Preparation

Both Shotgun and LC-MS analysis of biological samples for lipids requires extraction and reconstitution in the solvent system suitable for analysis.

Qualitative LC-MS or Shotgun lipid profiling

Evaluation of changing lipid profiles in samples collected under different conditions requires the establishment of the profile. This may involve the establishment of known lipid metabolite profile or identification of novel lipid metabolites, or a combination of both. This service is central to all discovery work.

Quantitative analysis of lipids

This generally involves targeted lipid analysis. Once a lipid profile is established, different samples can be compared to ascertain quantitative changes in the target lipids. For example, the entire range of prostaglandins and their metabolites (more than 150) can be quantitatively compared between normal, pre-malignant, and neoplastic tissue specimens. Alternatively, a single enzymatic product such as 12(S)-HETE or a combination of different pathway metabolites can be quantitatively compared. Lipid classes currently analyzed: Fatty acyl lipids (includes monohydroxy fatty acids, oxo fatty acids, epoxy fatty acids and their corresponding dihydroxy products, trihydroxy fatty acids (including lipoxins and resolvins), leukotrienes (including peptido leukotrienes), prostaglandins, thromboxanes, and their metabolites), sphingolipids, glycerophospholipids, glycerolipids, sterol lipids, and prenol lipids. However, there is no limit to the classes of lipids that can be quantified by the core.

LC-MS method development

Methods are already in place for the detection and quantification of most lipids listed above. However, sample-specific and/or any novel metabolite-specific methods require developmental work.

Data processing

Raw LC-MS data are processed and presented in the form easily understandable to the investigators. This service is free for the first 24 analyses. Data processing is chargeable for each additional 24 sample unit or part thereof. Data interpretation assistance is available as a collaboration, where necessary.

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Service Cost: A general summary of costs for services is available here here.

For detailed cost estimation, use our cost estimator

(The rates generated by the cost estimator are estimates only. Actual analytical costs will be known only after submission of the service request and acceptance of the quote by the user.)

All users must acknowledge NIH grant support for the Lipidomics core in their publications with the following text:

This study was supported in part by National Center for Research Resources, National Institutes of Health Grants S10RR027926 and S10OD032292.