The Lipidomics Core Facility conducts comprehensive analysis of cellular lipids that encompass fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, sterol lipids, and prenol lipids; in essence the study of the lipid metabolome. Structural diversity of the lipids stemming from the multifarious combination of structural entities like the fatty acids, glycerol, choline, glucosides, isoprenoid units, etc., makes the analysis of the lipidome extremely complex. Adding to this complexity, numerous pathways involved in the biosynthesis, turnover, and metabolism make the lipid pool of the cell highly dynamic. Analysis of such a complex and dynamic metabolome not only provides important clues about signaling cascades at the cellular level but also would be an invaluable tool to study pathophysiology. Such studies are possible with the advent of sophisticated mass spectrometric techniques. Lipidomics Core Facility of the Pathology Department at Wayne State University offers an extensive array of analytical services to investigate the lipidome. The services include sample preparation, lipid identification by mass spectrometry, shotgun and LC-MS lipid profiling, LC-MS lipid quantification, and training in sample preparation methods.
The Lipidomics Core Facility is supported by NIH/NCRR grant. All users must acknowledge NIH grant support for the Lipidomics core in their publications with the following text:
This study was supported in part by National Center for Research Resources, National Institutes of Health Grant S10RR027926